Glu-C, Sequencing Grade (S. aureus V8) is a serine protease that specifically cleaves at the C-terminus of either aspartic or glutamic acid residues. For Peptide Mapping and Protein Identification Enzyme Can Be Used Alone or with Other Proteases Item Suitable for in-Solution Digestion Reactions In ammonium bicarbonate and ammonium acetate, the enzyme specificity is higher at the glutamic residues. In phosphate buffers, cleavage occurs at the aspartic and glutamic residues. Glu-C activity is optimal in the pH range of 4.0â€"9.0. This sequencing-grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in solution but not recommended for in-gel digestions.
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